ISHI-Veg methods are developed using the collective expertise and experience of its members

ISHI-Veg Protocols

Method validation is a critical aspect of method development in ISHI-Veg. Performance criteria, such as analytical specificity, analytical sensitivity, selectivity, repeatability and reproducibility are measured to demonstrate that the method is fit for purpose. The methods follow ISHI-Veg best practices for PCR, ELISA and dilution-plating assays used in routine seed health testing. The best practice documents identify the number and types of controls necessary for a robust seed health test.

ISHI-Veg methods are reviewed at regular intervals and revised, if found necessary. Therefore, it is the responsibility of the user to check that the most recent version of the protocol is being applied.
Contact the ISF Secretariat if you would like to know more about these methods.


ISF cannot guarantee that labs following these protocols will obtain similar results. Many factors, such as staff skills, lab equipment and conditions, reagents and sampling methods can influence the results. Consequently, in case of any litigation ISF will not accept any liability on the use of these tests.


Pseudomonas savastanoi pv. phaseolicola

Xanthomonas axonopodis pv. phaseoli and X.a.p. var. fuscans


Phoma lingam

Xanthomonas campestris pv. campestris and X.c. pv. raphani (untreated seed)

Xanthomonas campestris pv. campestris (disinfested/disinfected seed)


Alternaria dauci (Blotter/agar)

Alternaria radicina (Blotter/agar)

A. radicina (A. radicina selective agar)

Xanthomonas hortorum pv. carotae

Celery, Celeriac

Septoria apiicola

Corn salad

Acidovorax valerianellae

Cucurbit species

Acidovorax citrulli  (Seed Extract qPCR)

Squash mosaic, Cucumber green mottle mosaic and Melon necrotic spot viruses


Lettuce mosaic virus


Acidovorax citrulli  (Sweat Box Grow-out)


Pea seed-borne mosaic virus and Pea early browning virus

Pseudomonas syringae pv. pisi



Tomato brown rugose fruit virus

Xanthomonas spp.


Verticillium dahliae


Clavibacter michiganensis subsp. michiganensis

Pepino mosaic virus


Tomato brown rugose fruit virus

Xanthomonas spp.